Lacto PAFI Techno Resources Corporation

Lactobacillus Pafi PDF Print E-mail
Written by Engr. Greg Sanchez Jr.   
Monday, 13 April 2009 12:04

The stronghold of probiotics

Even in the prehistoric times, fermented foods have long been the important constituents of the human diet. And in the recent centuries eating fermented foods is part of the eating customs of people from different parts of the world: Chileans gather in parks to eat empanadas; drink chicha, a sweet drink made from fermented grapes or apples; in Nigeria, one of their popular foods is hura (fermented milk with millet and hot pepper); in Vietnam, fermented fish sauce called nuoc mam is the main seasoning used to flavor dishes - special foods are also dipped in it.

       Bacteria  that  produce  lactic acid play major  roles  in  those fermented foods  eaten  by  our  ancestors,    which in  our  present times called “Lactobacillus”  that is attributed to produce  various  kinds  of  vitamins  and minerals   that   contributed   much   to achieve good health. It was discovered that ancient people who ate fermented foods had achieved long life span.

        Until, this strain of bacteria is used in preventing and treating various kinds of diseases, which is referred to as “Probiotics”.

        In the year 2000 was the  blossom of LactoPafi and Lactovitale probiotic health drinks food supplements in the Philippines that marked a remarkable dawn for the health-benefiting bacteria in the world of “Probiotics”.

          LactoPafi and Lactovitale probiotic health drinks food supplements are infused with eight strains of Lactobacillus they are: L. casei, L.paracasei, L. rhamnosus, L. Plantarum, L. brevis, L. zeae, L. lindneri, and L. perolens.

          Lactopafi and Lactovitale probiotic health drinks are cultured on nutrient-dense soy protein, molasses and fresh milk.

          Based on the deep-seated studies executed by many experts that beside their ability to normalize intestinal flora and to inhibit bad bacteria, they have the ability to enhance natural immune function in humans to fight against infectious diseases and observed to increase the production of interferon, a substance used in medication for cancer and tumor treatment and help reduce bad cholesterol that caused lifeshortening diseases – diabetes and heart attack.

          LactoPafi and Lactovitale are complete health drinks that would fill nutritional gap. These drinks contain a broad spectrum of vitamins, such as B1  or  thiamine  that  would  help  the absorption of pyruvic acid and help carbohydrates to release their energy,

          B2 or riboflavin serves in the maintenance of mucous membrane,  B3 or niacin works as coenzyme in the release of energy from nutrients and helps reduce level of cholesterol in the blood - used extensively in preventing and treating arteriosclerosis, B6 is necessary for the absorption and metabolism of amino acids and formation of red blood cells,  B12 for the formation of nucleoproteins, proteins and red blood cells and for functioning of the nervous system, and vitamin K for blood clotting.

          Furthermore, LactoPafi and Lactovitale contain 33 amino acids that play important role for the proper growth of children and in maintaining the body structure, among which are tryptophan, threonine, serine, glutamic acid, praline glycine, alanine, methionine, leucine and isoleucine, phenylalanine, and lysine.

          There were several studies conducted on the LactoPafi and Lactovitale, one was conducted by AIT (Asian Institute of Technology-Thailand).  It was found that there are 13 bacteria causing diseases which Lactopafi and Lactovitale can restrain the growth of.
           These bad bacteria are: Bacillus cereus which causes diarrhea, wound infection, blood poisoning, breast inflammation, and meningitis, which carried by human and animals; Bacillus circulans is accumulated through intestinal flora and contaminated food, causes epilepsy and swelling of the eyes, among others; Enterobacter cloacae, which causes sepsis, lower respiratory tract infection, UTI, infection of the heart valve, arthritis, acute inflammation of the bones, is accrued through water and some dairy products; Haemphilus influenzae, Klebsiela pneumonia, sinusitis, bronchitis, and ulceration of the soft nasal bones; Listeria monocytogenes is a source of infection in pregnancy and in the nervous system; Salmonella Typhimurium, Vibrio cholerae, Vibrio parahaemolyticus, Staphylococcus aureus and Escherichia coli or E. coli, both wild type and 0157:H7 can cause severe cramps and bloody diarrhea.
          Lactobacillus Pafi samples were sent to the United States of America for Analytical Laboratory at New Jersey Laboratories, a full Service Analytical Laboratory certified by United States Food and Drugs Administration (USFDA), USDA, NJ Department of Health, NJ Department. The analysis and examination were conducted by Joseph Gianella, M.S., Microbiology Laboratory Manager. There are 5,500 ,000 Lactobacillus plantarum per mL as the result of their test.
          In recent analytical and biological test in the year 2008, there are 73,000,000 of multi-strains of Lactobacilli per mL of Lactopafi and Lactovitale Probiotic Health Drink Food Supplements.

Known also as vitamin B complex, these are fragile, water-soluble substances, several of which are particularly important to carbohydrate metabolism.

Thiamine, or vitamin B1, a colorless, crystalline substance, acts as a catalyst in carbohydrate metabolism, enabling pyruvic acid to be absorbed and carbohydrates to release their energy. Thiamine also plays a role in the synthesis of nerve-regulating substances. Deficiency in thiamine causes beriberi, which is characterized by muscular weakness, swelling of the heart, and leg cramps and may in severe cases, lead to heart failure and death.

Riboflavin, or vitamin B2, like thiamine, serves as a coenzyme—one that must combine with a portion of another enzyme to be effective—in the metabolism of carbohydrates, fats, and, especially, respiratory proteins. It also serves in the maintenance of mucous membranes. Riboflavin deficiency may be complicated by a deficiency of other B vitamins; its symptoms, which are not as definite as those of a lack of thiamine, are skin lesions, especially around the nose and lips, and sensitivity to light.

Niacin, also known as nicotinic acid and vitamin B3, also works as a coenzyme in the release of energy from nutrients. A deficiency of niacin causes pellagra, the first symptom of which is a sunburn-like eruption that breaks out where the skin is exposed to sunlight. Later symptoms are a red and swollen tongue, diarrhea, mental confusion, irritability, and, when the central nervous system is affected, depression and mental disturbances.

Also our body makes niacin from the amino acid tryptophan. Mega doses of niacin have been used experimentally in the treatment of schizophrenia, although no experimental proof has been produced to show its efficacy. In large amounts it reduces levels of cholesterol in the blood, and it has been used extensively in preventing and treating arteriosclerosis. Large doses over long periods cause liver damage.

Pyridoxine, or vitamin B6, is necessary for the absorption and metabolism of amino acids. It also plays a role in the use of fats in the body and in the formation of red blood cells. Pyridoxine deficiency is characterized by skin disorders, cracks at the mouth corners, smooth tongue, convulsions, dizziness, nausea, anemia, and kidney stones.

Pyridoxine is needed in proportion to the amount of protein consumed.

Cobalamin, or vitamin B12, one of the most recently isolated vitamins, is necessary in minute amounts for the formation of nucleoproteins, proteins, and red blood cells, and for the functioning of the nervous system. Cobalamin deficiency is often due to the inability of the stomach to produce glycoprotein, which aids in the absorption of this vitamin. Pernicious anemia results with its characteristic symptoms of ineffective production of red blood cells, faulty myelin (nerve sheath) synthesis, and loss of epithelium (membrane lining) of the intestinal tract.

Folic acid, or folacin, is a coenzyme needed for forming body protein and hemoglobin. Recent investigations show that folic acid deficiency may be responsible for neural tube defects, a type of birth defect that results in severe brain or neurological disorders.

Folic acid is effective in the treatment of certain anemias and sprue. Folic acid is lost in foods stored at room temperature and during cooking. Unlike other water-soluble vitamins, folic acid is stored in the liver and need not be consumed daily.

Pantothenic acid, another B vitamin, plays a still-undefined role in the metabolism of proteins, carbohydrates, and fats. It is abundant in many foods and is manufactured by intestinal bacteria as well.

Biotin, a B vitamin that is also synthesized by intestinal bacteria and widespread in foods, plays a role in the formation of fatty acids and the release of energy from carbohydrates. Its deficiency in humans is unknown.

 Lactobacillus bacterium is known to produce

          This vitamin is necessary mainly for the coagulation of blood. It aids in forming prothrombin, an enzyme needed to produce fibrin for blood clotting. For a healthy adult, a normal diet and bacterial synthesis in the bowels usually are sufficient to supply the body with vitamin K and prothrombin. Digestive disturbances may lead to defective absorption of vitamin K and hence to mild disorders in blood clotting.

Lactopafi and Lactovitale contain  antioxidants

          These probiotic health drinks are infused with vitamins A and E from fresh milk, which are scientifically known as antioxidants to counter the potential harm of chemicals known as free radicals that can cause damage and make cells more vulnerable to cancer-causing substances.

Essential nutrients compounded in LactoPafi  and Lactovitale probiotic health drinks

One of the ingredients of Lactopafi is milk which consists of an abundance of the major nutrients to maintain good health, including fats, carbohydrates, proteins, minerals, and vitamins.

Cow’s milk typically contains about 3.5 to 5 percent fat, which is dispersed throughout the milk in globules. In addition to providing milk’s characteristic taste and texture, fat supplies vitamins A, D, E, and K, as well as certain fatty acids that the body cannot produce on its own.
 Lactose, a kind of sugar found only in milk, that makes up about 5 percent of milk’s content, lactose is a carbohydrate that is broken down by the body to supply energy. Infants digest lactose easily. But for adults, there are some who are milk intolerance; they lost some of their ability to digest this sugar, that when they drink milk, they often suffer gastric distress and diarrhea.

The most important protein in milk is casein, accounting for 80 percent of milk protein. Casein is a complete protein, meaning that it contains all of the essential amino acids, which the body cannot manufacture on its own. Casein molecules and globules of fat deflect light rays passing through milk, giving milk its opalescent appearance. Other proteins present in milk include albumin and globulin.

Milk contains many minerals, the most abundant of which are calcium and phosphorus, as well as smaller amounts of potassium, sodium, sulfur, aluminum, copper, iodine, manganese, and zinc.

Soybean extract

          One of the components of Lactopafi and Lactovitale probiotic health drinks is soybean extract which is known to have rich in protein.

          Protein is a macronutrient with a chemical structure containing carbon, hydrogen, oxygen, and nitrogen. It is the nitrogen that gives protein its unique properties.

          Dietary proteins are powerful compounds that build and repair body tissues, from hair and fingernails to muscles. In addition to maintaining the body’s structure, proteins speed up chemical reactions in the body, serve as chemical messengers, fight infection, and transport oxygen from the lungs to the body’s tissues.

          The body should break down protein from food into amino acids before it reshuffles them into new protein to build and rebuild tissue, including muscle. But, on the other hand, the healthful benefits brought by billions of probiotic bacteria in Lactopafi and Lactovitale, Lactobacilli bacteria break down soybean proteins into amino acids, which mean: ready to be absorbed by the body.

          Protein also keeps your immune system functioning up to par, helps carry nutrients throughout the body, has a hand in forming hormones, and is involved in important enzyme reactions such as digestion.

          Of the more than 20 amino acids our bodies require, eight (nine in some older adults and young children) cannot be made by the body in sufficient quantities to maintain health. These amino acids are considered essential and must be obtained from food. When we eat food rich in proteins, the digestive tract breaks this dietary protein into amino acids which are absorbed into the bloodstream and sent to the cells that need them, amino acids then recombine into the functional proteins our bodies need.

           Eight essential amino acids are needed to maintain health in humans: leucine, isoleucine, lysine, methionine, phenylalanine, theonine, tryptophan, and valine.

          Although amino acids work together to form body proteins, individual amino acids   have  specific  roles  to  play  in  the  body.  Certain  amino  acids,  such  as
tryptophan and tyrosine, are involved in the formation of chemical messengers called neurotransmitters for the brain and nervous system. Three amino acids (leucine, isoleucine, and valine) are constituents of muscle tissue.

          Evidence indicates that the form of a nutrient in foods is better absorbed than the form that comes in pills or powders. Calcium in milk and other dairy products, for example, is known to be better absorbed by the body than in pill form.

          Some people, especially in the United States and other developed countries, consume more protein than the body needs. Because extra amino acids cannot be stored for later use, the body destroys these amino acids and excretes their by-products. Marasmus and kwashiorkor, both life-threatening conditions, are the two most common forms of protein malnutrition.   

          For adults, the Recommended Dietary Allowance (RDA) for protein is 0.79 g per kg (0.36 g per lb) of body weight each day. For children and infants this RDA is doubled and tripled, respectively, because of their rapid growth.

          Some health conditions, such as illness, stress, and pregnancy and breast-feeding in women, place an enormous demand on the body as it builds tissue or fights infection, and these conditions require an increase in protein consumption. For example, a healthy woman normally needs 45 grams of protein each day. Experts recommend that a pregnant woman consume 55 grams of protein per day, and that a breast-feeding mother consume 65 grams to maintain health.

           A man of average size should eat 57 grams of protein daily. Infants and young children require relatively more protein than do adults to support their rapid development. A three-month-old infant requires about 13 grams of protein daily, and a four-year-old child requires about 22 grams. Once in adolescence, sex hormone differences cause boys to develop more muscle and bone than girls; as a result, the protein needs of adolescent boys are higher than those of girls.

Sparks your memory

Lactopafi and Lactovitale probiotic food supplements are abounding with complete standard amino acids.

          Known as the standard, or alpha, amino acids, they comprise alanine, arginine, asparagine, aspartic acid, cysteine, glutamic acid, glutamine, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, and valine

          Animal proteins and soy protein are of higher quality because they contain all the essential amino acids in larger amounts and better proportions. In plants, amino acids exist in smaller concentrations. For the body to make proteins properly, all 20 amino acids must be present at the same time.

          One amino that is believed to increase memory is tryptophan. According to study, tryptophan is transported to the brain, where it is broken down by enzymes to produce serotonin. In the process of neurotransmission, serotonin is transferred  from one nerve cell, or neuron, to another, triggering an electrical impulse that stimulates or inhibits cell activity as needed. Serotonin is then reabsorbed by the first neuron, in a process known as reuptake, where it is recycled and used again or converted into an inactive chemical form and excreted.

          Serotonin is a neurotransmitter, or chemical that transmits messages across the synapses, or gaps, between adjacent cells. Among its many functions, serotonin is released from blood cells called platelets to activate blood vessel constriction and blood clotting. In the gastrointestinal tract, serotonin inhibits gastric acid production and stimulates muscle contraction in the intestinal wall. Its functions in the central  nervous  system  and  effects  on  human  behavior -Including  mood, memory, and appetite control-have been the subject of a great deal of research. This intensive study of serotonin has revealed important knowledge about the serotonin-related cause and treatment of many illnesses.

Facts about Interferon

          Lactopafi and Lactovitale health drinks are compounded with the multi-strains of lactobacilli bacteria which according to study by  some experts that one of its strains, Lactobacillus brevis  bacterium, has the ability to produce interferon, a substance that causes cells to produce active antiviral proteins. Interferon is used as medication on certain cancers and tumors.

          Viruses cannot be treated with antibiotics, but the body’s immune system has many natural defenses against virus infections. Infected cells produce interferons (soluble components that are largely responsible for regulating the immune response), which can signal adjacent uninfected cells to mount their defenses, enabling uninfected cells to impair virus replication.

          Interferon may be grouped into three categories. Alpha (leukocyte) interferons are made by white blood cells, beta (fibroblast) interferons by skin cells, and gamma (immune) interferons by lymphocytes after stimulation by antigens.

Interferon as treatment
           Synthetic forms of interferon (a natural immune chemical that regulates immune responses) reduce the frequency and severity of relapses and slow the accumulation of disability over time of Multi Sclerosis – a chronic, unpredictable, and often progressive disease of the central nervous system that attacks and destroys tissues in the brain and spinal cord.

Potential skin cancer treatment studies now underway include the use of
 the drugs interleukin-2 and interferon. A vaccine to reduce the recurrence
 of melanoma has shown promise in early tests.
The only available treatment for hepatitis B is interferon, a drug that is
 effective in only 35 to 40 percent of patients treated.
Interferon is also used in immunotherapy. It slows down the growth of tumor cells in some patients, and stimulates the immune system to attack cancerous cells.

The discovery of interferon
          Interferon had been known to its significance to shield the human body against the invading viruses when Virologists Alick Isaacs (Scottish) and Jean Lindenmann (Swiss) discover interferons in the year 1957. In chick embryo cells interferons were found to block further viral infection of body cells. According to their study, that when a virus invades a cell, the cell produces interferon, which then induces uninfected cells to make a protein that prevents the virus from multiplying. Almost any cell in the body can make interferon, which seems to act as the first line of defense against viral pathogens, because it is produced very quickly. Interferon production starts within hours of infection whereas antibody production takes several days. Interferon is also thought to trigger other defense mechanisms. Interferons play a role in the body’s most important defenses against viruses, and that they help fight bacteria and other disease-causing agents.
          Interferon is a group of antiviral proteins produced by animals, including humans, in response to viral infection. Interferons help the body fight further infection.

Scientific study supports
LACTOPAFI benefits

          A good probiotic supplement will contain million of live bacteria to bolster and replenish levels of health by promoting good bugs in our digestive tract.
         All these good effects have been documented. Now, conclusions of a scientific study here in Cebu support more of its claims to potency.
          Such was the finding of a recent study conducted by the Pathology Department of the Vicente Sotto Memorial Medical Center (VSMMC) as it tested four (4) strains of bacteria that cause diseases in humans against Lactobacillus Plantarum in Lactopafi Probiotic Food Supplement.
The test
          To properly investigate the effectiveness of the “good bug” in Lactopafi health drink, a laboratory setting was established.
          The test was to introduce Lactopafi to four different types of illness-causing bacteria to see whether it can combat the growth and eventually defeat the “bad bugs”.
          Petri dishes containing the following disease-causing bacteria were prepared: Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Staphylococcus aureus.

          One, two and five milliliters of Lactopafi health drink were introduced into these environments. A control was set up, this did not contain Lactopafi.
          Effects were then observed after contact times of 6, 18 and 30 hours.
          The control group (without Lactopafi) revealed consistent uninhibited growth of the four bacteria in six hours.
S. aureus
          S. aureus proved to be a rather strong bacterium compared to the other three. When various amounts of Lactopafi were introduced, in the presence of growth was still noted six and 18 hours after contact time. However, thirty hours after, the good triumphed over the bad bacteria. S. aureus stopped growing and was eventually defeated.
          S. aureus is a spherical bacterium and causes food poisoning. Onset symptoms is usually rapid and in many cases acute, depending on individual susceptibility to the toxin, the amount of contaminated food eaten, the amount of toxin in the food ingested, and the general health of the victim. (LLP)
K. pneumoniae
          Lactopafi when introduced into the K. pneumoniae setup also defeated the bad bug after only 6 hours of contact time. Eighteen and thirty hours after, no further increase in the bacteria was noted.

          Clinical Manifistations of K. Pneumoniae include pneumonia, bacteremia, wound infections, urinary tract infections. It accounts for a small percentage of pneumonia cases but extensive damage produced by organism results in high case fatality rates (up to 90 % of untreated patients.)
P. aeruginosa
          Lactopafi was introduced into the P. eariginosa environment. After only six hours from contact time, no further growth was noted. It effectively halted the bacteria from spreading 18 and 30 hours after.
          Pseudomonas aeruginosa, is a frequent cause of nosocomial infections such as pneunomia, urinary tract infections (UTIs), and bacteremia. Pseudomonal infections are complicated and can be life threatening.
E. coli
          One, two and five milliliter drops of Lactopafi were separately added into the E. coli set-up.
The test revealed that no growth in the bacteria was noted after only its six hours contact time. No further growth was observed 18 and 30 hours after. Lactopafi effectively inhibited E.coli.
          One, two and five milliliter drops of Lactopafi were separately added into the E. coli set-up. The test revealed that no growth in the bacteria was noted after only its six hours contact time. No further growth was observed 18 and 30 hours after. Lactopafi effectively inhibited E.coli.
          Escherichia coli or E. coli causes severe cramps and is the leading causes of bloody diarrhea. People who are infected with E.coli are very contagious. Infection can be acquired through eating undercooked ground beef where the inside is pink), drinking contaminated (impure) water, drinking unpasteurized (raw) milk, or working with cattle.


Last Updated on Monday, 13 April 2009 12:30
You are here  : Home Lactobacillus